Effects of Indoor Dust Exposure on Lung Cells: Association of Chemical Composition with Phenotypic and Lipid Changes in a 3D Lung Cancer Cell Model

Indoor dust is a key contributor to the global human exposome in urban areas since the population develops most of its activities in private and public buildings. To gain insight into the health risks associated with this chronic exposure, it is necessary to characterize the chemical composition of dust and understand its biological impacts using reliable physiological models. The present study investigated the biological effects of chemically characterized indoor dust extracts using three-dimensional (3D) lung cancer cell cultures combining phenotypic and lipidomic analyses. Apart from the assessment of cell viability, reactive oxygen species (ROS) induction, and interleukin-8 release, lipidomics was applied to capture the main lipid changes induced as a cellular response to the extracted dust compounds. The application of chemometric tools enabled the finding of associations between chemical compounds present in dust and lipidic and phenotypic profiles in the cells. This study contributes to a better understanding of the toxicity mechanisms associated with exposure to chemical pollutants present in indoor dust.