Potential anti-inflammatory biomarkers from Myrtaceae essential oils revealed by untargeted metabolomics

Many species from Myrtaceae have traditionally been used in traditional medicine as anti-inflammatory, antimicrobial, antidiarrheal, antioxidant and antirheumatic, besides in blood cholesterol reduction. In the present work, the anti-inflammatory activity of essential oils from eighteen Myrtaceae spp. were evaluated according to their ex-vivo anti-inflammatory activity in human blood, and the corresponding biomarkers were determined using untargeted metabolomics data and multivariate data analysis. From these studied species, six displayed anti-inflammatory activity with percentage rates of inhibition of PGE2 release above 70%. Caryophyllene oxide (1), humulene epoxide II (2), beta-selinene (3), alpha-amorphene (4), alpha-selinene (5), germacrene A (6), beta-bisabolene (7), alpha-muurolene (8), alpha-humulene (9), beta-gurjunene (10), myrcene (11), beta-elemene (12), alpha-cadinol (13), alpha-copaene (14), E-nerolidol (15) and ledol (16) were annotated as potential anti-inflammatory biomarkers. The results obtained in this study point to essential oils from species of the Myrtaceae family as a rich source of anti-inflammatory agents. [GRAPHICS]