Artificial nucleotide codons for enzymatic DNA synthesis

Herein, we report the high-yielding solid-phase synthesis of unmodified and chemically modified trinucleotide triphosphates (dN3TPs). These synthetic codons can be used for enzymatic DNA synthesis provided their scaffold is stabilized with phosphorothioate units. Enzymatic synthesis with three rather than one letter nucleotides will be useful to produce xenonucleic acids (XNAs) and for in vitro selection of modified functional nucleic acids. Synthesis three-by-three: trinucleotide triphosphates can be used for enzymatic DNA synthesis provided their backbone is stabilized with chemical modifications.