Potential role of AgNPs within wastewater in deteriorating sludge floc structure and settleability during activated sludge process: Filamentous bacteria and quorum sensing.

Excellent sludge floc structure and settleability are essential to maintain the process stability and excellent effluent quality during the activated sludge process. The underlying effect of silver nanoparticles (AgNPs) within wastewater on sludge floc structure and settleability is still unclear. The potential role of AgNPs in promoting filamentous bacterial proliferation and deteriorating sludge floc structure and settleability based on quorum sensing (QS) were investigated in this study. The results indicated that N-acyl homoserine lactose (AHL) concentration sharply increased from 23.56 to 108.41 ng/g VSS in the sequencing batch reactor with 1 mg/L AgNPs. AgNPs strengthened communication between filamentous bacteria, which triggered the filamentous bacterial QS system involving the synthetic gene hdtS and sensing genes traR and lasR. Filamentous bacterial proliferation was promoted by the triggered QS via positively regulating its cell cycle progression including chromosomal replication and divisome formation. In addition, extracellular protein production was obviously increased from 43.56 to 97.91 mg/g VSS through QS by regulating arginine and tyrosine secretion during filamentous bacterial proliferation under 1 mg/L AgNPs condition, which led to an increase in the negative charge and hydrophily at the cell surface. AgNPs resulted in an obvious increase in the surface energy barrier (WT) between bacteria. The change in the physicochemical properties of extracellular polymeric substance (EPS) induced by QS among filamentous bacteria obviously inhibited bacterial aggregation between filamentous bacteria and floc-forming bacteria under AgNPs condition, thus resulting in serious deterioration of the sludge floc structure and settleability. This study provided new insights into the microcosmic mechanism for the effect of AgNPs on sludge floc structure and settleability.