Temporal and spatial variations in the physical and chemical properties of anaerobic granular sludge within a Pilot Spiral Symmetry Stream Anaerobic Bioreactor

Anaerobic granular sludge (AGS) determines the high performance of the bioreactor. To study the regionalization of granular sludge in the bioreactor, a Pilot Spiral Symmetry Stream Anaerobic Bioreactor (P-SSSAB) was established over 216 days, divided into three zones (I, II, and III) from bottom to top. AGS at the bottom of PSSSAB had a higher porosity (60.35 %-83.27 %) and more suitable settling velocity (60 m/h) when the particle size range was 1.0-2.0 mm. This proved the better metabolic activity and superior settling performance in zone I than in zones II and III. In addition, the elemental composition of AGS in various zones was analyzed. The relative content of iron (5.66 %, 3.36 %, and 1.38 %, respectively) and sulfur (2.47 %, 2.19 %, and 1.49 %, respectively) in zone I, II, and III tended to decrease with the height of P-SSSAB. This also verified the better mass transfer performance and operational stability in lower zone than in upper zone. However, the monitoring of bed temperature in various zones revealed that the microbial activity in zone I was 6.7 x 10-12 ti 3.5 x 10-2 times and 1.8 x 10-15 ti 1.4 x 10-3 times that in zones II and III, respectively, which indicated that the unit activity of AGS in zone I was the worst. It indicated that AGS in lower zone had poor unit activity but had the highest unit capacity due to the high sludge concentration. Besides, the unit capacity of the upper zone was too weak to produce enough alkalinity to neutralize acid produced by excessive hydrolysis and acidification in lower zone, resulting in the worst treatment efficiency of the upper zone. Therefore, temperature and concentration ratios under various spatial distributions in bioreactors are vital to the overall sewage treatment stability and efficiency of bioreactors in actual engineering applications.