Immobilized Cellulase on NH 2 -MIL-88(Fe) and Its Performance as a Biocatalyst

To broaden pH range and improve thermal stability, reusability, storage stability, and organic solvent tolerance of natural enzymes, a magnetic material (NH 2 -MIL-88(Fe)) was synthesized as a new material to immobilize cellulase. The results showed that the optimal temperature and pH of cellulase immobilized on NH 2 -MIL-88(Fe) showed a wider range compared to free cellulase, and 74% and 83% of the initial activity could be retained after 10 cycles and storage for 49 days, respectively. Moreover, the tolerance for organic solvents was improved compared with free enzyme. The reducing sugar yields from sodium carboxymethylcellulose (CMC) and corn cob hydrolyzed with cellulase immobilized on NH 2 -MIL-88(Fe) were higher than observed with the free enzyme, which demonstrated the better biocatalytic performance of cellulase immobilized on NH 2 -MIL-88(Fe).