Untargeted metabolomics analysis reveals the efficacy of Xinsuning capsules in ameliorating arrhythmias in rats

Background: Xinsuning capsules is a safe and effective drug against phlegm-heat-disturbedarrhythmia (a type of rapid arrhythmia in traditional Chinese medicine. The clinical manifestations include chest tightness, irritability, vomiting, insomnia, and dreaminess) with no noticeable adverse effects. However, the molecular biological basis of Xinsuningcapsules in the treatment of arrhythmia is relatively unexplored. Methods: In order toassessthe antiarrhythmic effects of Xinsuning capsules, a rat arrhythmia model was constructedbyinjecting barium chloride into the tail vein. Metabolomic analysis was performedbyUHPLC/Q-Exactive-Orbitrap mass spectrometry. The selection of differential variables inthemetabolic data were necessitated fold change >= 1.5 or fold change <= 0.67, together withP< 0.05. Results: Barium chloride-induced arrhythmias in rats were significantly delayedinbeginning and cut short in duration, according to electrocardiogrammonitoring. Left ventricles of rats exhibitied significantly higher Na+ -K + -ATPase activity after takingXinsuning capsules compared to the model group. Metabolomic analysis showedthat Xinsuning capsules could regulate arachidonic acid, prostaglandin F2a, 15-HETE, 15-HPETE, 12-HETE, 12(13)-DHOME, LTB4, inosine, and hypoxanthine. Thesemetabolomics mainly involved arachidonic acid metabolism, unsaturated fat acidbiosynthenosis, and purine metabolism. Conclusion: The therapeutic effects of Xinsuningcapsules against arrhythmia had been investigated with metabolomics, providing the basisfor further comprehensive research.