MiR-100-5p transfected MSCs-derived exosomes can suppress NSCLC progression via PI3K-AKT-mTOR

Objectives: Exosomes are highly implicated in lung cancer and are capable of transferring therapeutic miRNAs.Methods: Database analysis was performed to screen the probable miRNA involved in non-small cell lung cancer (NSCLC). The levels of miR-100-5p in NSCLC cells and tissues were evaluated. The mechanism by which MSC-derived exosomes mediate the delivery of miR-100-5p in NSCLC cells was explored in vitro. The therapeutic effect and safety of miR-100-5p-containing MSC-derived exosomes in nude mice were assessed.Results: MiR-100-5p was significantly downregulated in NSCLC. Transfer of miR-100-5p via MSCs-derived exosomes inhibited NSCLC progression by the PI3K-AKT-mTOR pathway. No obvious toxic effects were observed in mice.Conclusions: MSCs-derived exosome-transfected miR-100-5p inhibits NSCLC progression via PI3K-AKT-mTOR, providing a promising diagnostic biomarker and therapeutic target of NSCLC.