Solution and solid-state ³³S NMR studies of ³³S-labeled taurine

Sulfur-33(S-33) stable-isotope labeled taurine, 2-aminoethanesulfonic acid, has been synthesized, and a series of solution and solid-state S-33 nuclear magnetic resonance (NMR) experiments at 14.1 and 18.8 T, respectively, have been carried out at room temperature. The single peak of a solution S-33 NMR spectrum in 0.1-mM [S-33]-taurine in D2O can be observed with the signal-to-noise (S/N) ratio of 9 in 40,000 scans, which paves the way toward in vivo analysis of pharmacokinetics and metabolism of S-33-labeled taurine. Undistorted magic-angle-spinning (MAS) and static S-33 NMR spectra of polycrystalline [S-33]-taurine are observed with sufficient S/N ratios for analysis, and the magnitudes of S-33 EFG and CS tensors can be obtained.