NIRS for vicine and convicine content of faba bean seed allowed GWAS to prepare for marker-assisted adjustment of seed quality of German winter faba beans
JOURNAL FUR KULTURPFLANZEN(2022)
摘要
GWAS was applied to the antinutritive compounds vicine and convicine (V, C) in winter faba bean. V, C and V + C data for 189 inbred lines (five environments) were predicted by NIRS. These lines do not carry the strong "vc-" allele (locus VC1). Lab data for 646 samples enabled our NIRS calibration, which performed well for V and V + C yet poor for C. Heritability was high (0.911; 0.868) for V and V + C and lower for C (0.737). From the 2542 mapped SNPs, 47 were significantly associated with V and one with V + C. Four SNPs mapped near to the VC1 locus and were significant for V. Seemingly, non-"vc-" alleles at that locus contributed to V variation. Marker-assisted breeding with this germplasm can reduce the V + C content to about 0.44%, compared to the current lowest line with 0.55%. Further research will show inasmuch this can serve agronomy and breeding.
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关键词
faba bean seed,seed quality,marker-assisted
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