Marnyiella aurantia, gen. nov., sp. nov., a novel bacterial species of the family Weeksellaceae that could produce flexirubin type pigments

Two Gram- stain- negative, non- spore- forming, rod- shaped, and obligately aerobic bacteria, designated strains CX- 624(T) and cx- 311, were isolated from soil samples in Qinghai Province, China. The two strains grew best at 28 C-degrees on the plate with Tryptone soya agar (TSA). Cells formed circular, convex, translucent, smooth, and orange colonies with approximately 1.0 mm diameter after 2 days of incubation on TSA at 28 C-degrees. The strains were oxidase- negative and catalase- positive. The predominant cellular fatty acids were iso- C-15: 0 and anteiso- C-15:0, and major polar lipids included phosphatidylethanolamine, an unidentified aminophospholipid, four unidentified lipids and an aminolipid. MK- 6 was the sole menaquinone in strain CX-624(T). Comparative analysis of the nearly full- length 16S rRNA gene sequences showed strains CX- 624(T) and cx- 311 were member of the family Weeksellaceae, with the highest similarity to Kaistella haifensis H38(T) (96.66 %), Epilithonimonas pallida DSM 18015(T) (96.59 %), and Chryseobacterium gambrini DSM 18014(T) (96.53 %). Both phylogenetic analysis of the 16S rRNA gene and 177 core genes revealed that strains CX- 624(T) and cx- 311 formed an independent clade. Average nucleotide identity values (< 72.64 %), average amino- acid identity values (<72.61 %) and digital DNA-DNA hybridization (< 21.10 %) indicated that the strains CX- 624(T) and cx- 311 should constitute a novel genus. The DNA G+C contents of strains CX- 624(T) and cx- 311 were 43.0 mol% and 42.7 mol%. According to the data obtained in this study, strain CX- 624(T) represents a novel species belonging to a novel genus of the Weeksellaceae, for which the name Marnyiella aurantia gen. nov., sp. nov. is proposed. The type strain is CX- 624(T )(=GDMCC 1.1714(T) = JCM 33925(T)).