A one-pot method based on rolling circle amplification and light‐activated CRISPR/Cas12a reaction for simple and highly sensitive detection of Staphylococcus aureus

Qian Wu,Song Jiang, Yong Huang, Lijun Zhang, Zongying Li,Yongbin Hou,Juan Zhang,Yuwei Wang, Chuanlin Zhu, Dandan Zhou,Weixian Chen,Dandan Li

Chemical Engineering Journal（2023）

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摘要

•A light-activated rolling circle amplification-triggered CRISPR/Cas12a method is developed.•The rolling circle amplification and Cas12a reactions can be separated in the time dimension.•This method shows higher sensitivity compared with the conventional one-pot assay.•Testing of 120 real samples demonstrates the good application capacity of this method.

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关键词

One-pot method,Light-activated CRISPR/Cas12a,Rolling circle amplification,Staphylococcus aureus

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