Microwave-assisted aqueous two-phase extraction of polysaccharides from Hippophae rhamnoide L.: Modeling, characterization and hypoglycemic activity
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES(2024)
摘要
Natural polysaccharides are concerned for their high biological activity and low toxicity. Two kinds of polysaccharides were extracted from Hippophae rhamnoide L. by microwave-assisted aqueous two-phase system. Under the optimal conditions predicted by RSM model (K2HPO4/ethanol (18.93 %/28.29 %), liquid to material ratio 77 mL/g, power 625 W and temperature 61 degrees C), the yield of total polysaccharides reached 35.91 +/- 0.76 %. Moreover, the polysaccharides extraction was well fitted to the Weibull model. After purification by Sepharose6B, the polysaccharides from top phase (PHTP, purity of 81.44 +/- 1.25 %) and bottom phase (PHBP, purity of 88.85 +/- 1.40 %) were obtained. GC, FT-IR, methylation and NMR analyses confirmed the backbone of PHTP was composed of a repeated unit -> 4)-beta-D-Glcp-(1 -> 2)-alpha-L-Rhap-(1 -> 4)-beta-D-Galp-(1 -> 4)-alpha-D-GalAp-(1 -> 3)-alpha-LAraf-(1 -> 3)-alpha-D-Manp-(1 ->, while the repeated unit in PHBP was -> 3)-alpha-L-Araf-(1 -> 2)-alpha-L-Rhap-(1 -> 4)-beta-D-Glcp-(1 -> 3)-alpha-D-Manp-(1 -> 4)-beta-D-Galp-(1 -> 4)-alpha-D-GalAp-(1 ->. Compared with PHTP (6.46 x 106 g/mol), PHBP with relatively low molecular weight (8.2 x 105 g/mol) exhibited the smaller particle size, better water solubility, thermal and rheological property, stronger anti-glycosylation and alpha-amylase inhibitory effects. Moreover, PHTP and PHBP displayed a reversible inhibition on alpha-amylase in a competitive manner. This study provides a high-efficient and eco-friendly method for polysaccharides extraction, and lays a foundation for sea buckthorn polysaccharides as potential therapeutic agents in preventing and ameliorating diabetes.
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关键词
Microwave-assisted aqueous two-phase extrac-tion,Characterization,Hypoglycemic activity
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