A marker-free genome editing method in S. pombe using the delitto perfetto approach.

The fission yeast, like budding yeast, offer an easy manipulation of their genome, despite their distinct biology. Most tools available in budding yeast are also available in fission yeast in versions taking into account the features of each organism. The is a powerful approach, initially developed in , for site-directed mutagenesis. Here, we present an adaptation of the approach to manipulation and demonstrate its applicability for a rapid, marker-free and efficient site-directed mutagenesis and N-terminal tagging of nonessential genes in fission yeast.