Determination of H2O2 and its antioxidant activity by BCM@Au NPs ratiometric SERS sensor

As a reactive oxygen species (ROS), excessive production of H2O2 contributes to the development of several diseases such as, inflammation, cancer, and respiratory diseases. Supplementation with endogenous or exoge-nous antioxidants can scavenge ROS and reduce the oxidation of cellular molecules, thus alleviating the gen-eration of diseases. Therefore, the determination of H2O2 content and its antioxidant activity is of great importance in disease diagnosis and treatment. In this paper, a ratiometric SERS sensor with a flexible cellulose membrane was designed for quantitative detection of H2O2 and assessment of antioxidant activity. First, gold seeds were reduced on bacterial cellulose membrane (BCM) and Au NPs were smoothly deposited on the bac-terial cellulose membrane (BCM) using halides to reduce the reduction potential in the growth solution to form a flexible BCM@Au NPs SERS substrate. Afterwards, the oxidation of H2O2 was used to convert 3-mercaptophenyl-boronic acid (3-MPBA) to the corresponding phenol form 3-hydroxyphenylethanol (3-HTP). The change of substance resulted in a good linear relationship between the intensity ratio corresponding to the two Raman shifts of 881 cm-1 and 995 cm-1 and the H2O2 concentration with a detection limit of 0.0186 mu M. This opens up a new method for the detection of H2O2 with high sensitivity and accuracy. In addition, this SERS platform was successfully used for the determination of antioxidant activity. It is promising to be applied to disease diagnosis and efficacy evaluation.