Glycerol production through TPI1 defective Kluyveromyces marxianus at high temperature with glucose, fructose, and xylose as feedstock

•TIP1 defective Kluyveromyces marxianus was constructed in this study.•TIP1 defective K. marxianus could grow with glucose as solo carbon source.•Glycerol was produced with this strain from glucose, xylose and fructose at 42 °C.•The processes were performed under aerobic condition with no byproduct accumulation.•A platform for bio-based glycerol and glycerol derivatives was developed.