Fluorescent composite based on peptide nanotubes activating coumarin 6 for sensitive detection of new coccine in food samples

New coccine (NC), as a kind of common colorant, has been frequently used in our daily life. Herein, the fluo-rescent composite (PNTs@C6) prepared by the hydrophobic non-covalent interaction between peptide nanotubes and coumarin 6 (C6) was designed for the determination of NC. Due to the activation of C6 by peptide nanotubes, the composite exhibits strong green fluorescence emission, which can be selectively quenched by NC through the inner filter effect. Therefore, a new fluorescent method based on the PNTs@C6 composite for NC detection was constructed. Under optimal conditions, the fluorescence quenching of the sensor exhibits a good linear rela-tionship with the concentration of NC in the range of 0.01-10 mu M and the limit of detection is 3.6 nM. Furthermore, the strategy shows simplicity, rapid response and high selectivity and has been successfully applied to the detection of NC in food samples.