Dual suppression amplification based on resonance energy transfer and enzymatic biocatalytic precipitation for IgG electrochemiluminescence ultrasensitive assay
Sensors and Actuators B: Chemical(2023)
摘要
Due to the necessity of ultrasensitive bioassays in life science, electrochemiluminescence (ECL) methodologies coupled with amplification strategies has drawn great attention recently. Herein, a versatile and robust dual suppression-based signal amplification strategy was proposed for IgG specifically ultrasensitive detection. A sandwich ECL sensor was constructed by employing Ru(bpy)32+ adsorbed on silica microspheres (Ru@SiO2) as luminophor. Fe3O4 nanoparticles (Fe3O4 NPs) were introduced on sensing platform through an antigen-antibody affinity, which played double roles. As an acceptor of resonance energy transfer (RET), Fe3O4 NPs remarkably quenched the ECL signal of Ru@SiO2. Meanwhile, Fe3O4 NPs, as a typical peroxidase-mimicking enzyme, catalyzed H2O2 to produce insoluble product based on enzymatic biocatalytic precipitation (BCP). As such, an efficient dual suppressive ECL platform has been achieved by the integration of ECL-RET and BCP techniques, which has been applied to ultrasensitively detect IgG with a linear range and limit of detection of 10 fg/mL to 1 μg/mL and 4.9 fg/mL, respectively. The new integrated system with RET and BCP may provide a potential promising in the development of highly efficient ECL systems for clinical diagnosis.
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关键词
Electrochemiluminescence,Fe3O4 nanoparticles,Resonance energy transfer,Enzymatic biocatalytic precipitation,Dual suppression
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