New genetic markers for 100% arabica coffee demonstrate high discriminatory potential for InDel-HRM-based coffee authentication

Edson Mario de Andrade Silva,Isabel Samila Lima Castro,Ananda Pereira Aguilar,Eveline Teixeira Caixeta,Tiago Antônio de Oliveira Mendes

Food Research International（2023）

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摘要

•InDel markers distinguish C. arabica and C. canephora genes.•PCR amplicon size discriminates C. arabica and C. canephora.•InDel markers reveal Coffea species diversity via clustering.•InDel-HRM markers detect Coffea spp. in beans with 0.5% sensitivity.

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关键词

Coffee beans, Quality control, Amplicon Size, Genotype Confidence Percentages, Food quality

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