A Single-Nucleotide Resolution Capillary Gel Electrophoresis Workflow for Poly(a) Tail Characterization in the Development of Mrna Therapeutics and Vaccines.

Deanna Di Grandi, Daniel M. Dayeh,Keerat Kaur, Yizhuo Chen, Steven Henderson,Youmi Moon, Arijit Bhowmick,Peter M. Ihnat,Yue Fu,Kathir Muthusamy,Nisha Palackal,Erica A. Pyles

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS（2023）

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关键词

In vitro transcribed messenger RNA (IVT mRNA),Poly(A) tail,Capillary gel electrophoresis (CGE),Single-nucleotide resolution,Ion-pair reversed-phase liquid chromatography (IPRP-LC),Anion exchange liquid chromatography (AEX-LC)

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