Anti-leishmanial and cytotoxic compounds isolated from marine sponge Hemimycale sp.

The methanolic extract of the marine sponge . yielded two new compounds; 1-(2'-methyl heptadecyl) phenol () and a new pyrazole derivative; 4-(hydroxymethyl)-1-pyrazol-3-ol (), together with previously isolated (2')-2'-hydroxy-N-((2,3,4)-1,3,4-trihydroxy-16-methylpentadecan-2-yl)docosanamide (), cholesterol (), 5, 8-dioxycholest-6-en-3-ol () and 3-acetylsesterstatin 3 (), which were firstly reported from family Hymedesmiidae. Their structure elucidation was based on extensive nuclear magnetic resonance spectroscopy and high resolution-electrospray ionization-mass spectrometry. The isolated compounds were evaluated for their anti-leishmanial and cytotoxic activities. Compound showed remarkable anti-leishmanial activity with IC value of 15.8 ± 0.92 µg/mL comparable with the standard miltefosine (IC = 3.2 ± 0.07 µg/mL), while compound exhibited noteworthy cytotoxicity against A594 cell line with IC value of 29.6 ± 1.68 µg/mL compared to etoposide (IC = 10.9 ± 1.30 µg/mL).