Stable and efficient biopolymeric nanocompozite of candida antarctica lipase b

The catalytic performance of various biocatalysts obtained by the adsorption of lipase B from Candida antarctica (CaL-B) onto and into polyvinyl alcohol (PVA) and polylactic acid (PLA) nanofibers were tested in the kinetic resolution of racemic 1-benzo[b]thiophen-2-yl-ethanol by transesterification. Best performance regarding reaction velocity and selectivity was registered for CaL-B adsorbed onto PLA nanofibers. The high operational stability of this biocatalyst was confirmed in recycling experiments, after 5 cycles the biocatalyst maintained 86.6% of its initial activity. The optimal process parameters in continuous flow mode also were established.