Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety

The rapid and highly sensitive detection of contaminants in foods is an important strategy to prevent food safety incidents. DNA Walker is a dynamic nano -machine with a programmable structure and function. It can be moved along the designed track interface driven by free energy to carry out continuous mechanical displacement. The nuclease powered DNA walker has excellent signal amplification capabilities and reaction kinetics, and the DNA walker -based biosensor combined with aptamer has been widely used in food safety detection. This review summarized the construction strategies of nuclease powered DNA walker and its application in food safety testing, and its further prospects were also discussed.