Anti-inflammatory probiotic Lactiplantibacillus plantarum HF05 screening from Qula: Genomic analysis and alleviating effect on intestinal inflammation

The use of probiotics for their anti-inflammatory potential is an area with limited research, and there is currently limited understanding of the connection between the genotype and phenotype of the majority of probiotics. In this study, Lactiplantibacillus plantarum HF05 with prominent anti-inflammatory effects and excellent probiotic characteristics was screened from Qula by combining RAW264.7 inflammatory cell model screening with in vitro comparison of probiotic properties. Through whole genome analysis, we found that the HF05 genome encoded a large number of environmental adaptation, adhesion, colonization, anti-inflammatory and antioxidant-related genes. The in vitro experiments demonstrated that HF05 inhibited pro-inflammatory cytokine release in RAW264.7 inflammatory cells. Furthermore, we found that prophylactic intake of HF05 alleviated intestinal inflammation and oxidative damage in mice with acute colitis and inhibited the value-added of enteropathogenic bacteria (Escherichia coli, Enterococcus faecalis, Staphylococcus xylosus) and reversed the depletion of Lactobacillus (Lactobacillus johnsonii, Lactobacillus intestinalis). Collectively, we demonstrated that HF05 can serve as a new probiotic for the relief of intestinal inflammation.