Mixed-mode column allows simple direct coupling with immobilized enzymatic reactor for on-line protein digestion

Zuzana Vosáhlová-Kadlecová,Martin Gilar,Katarína Molnárová,Petr Kozlík,Květa Kalíková

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences（2023）

引用 0|浏览2

暂无评分

摘要

•Rapid method for on-line tryptic digestion of proteins was developed.•Mixed-mode chromatography column was used for separation of protein digests.•Trypsin activity under various conditions was assessed with BAPNA as a substrate.•Off-line digestion via spin column was compared to on-line trypsin columns.•For cys–cys proteins on-line digestion provided higher sequence coverage.

查看译文

关键词

Trypsin digestion,On-line protein digestion,Mass spectrometry,Immobilized enzymatic reactor,Mixed-mode column

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要