Trends of enterovirus D68 concentrations in wastewater in two California communities parallel trends of statewide confirmed cases, February 2021-April 2023

In this retrospective study, enterovirus D68 (EVD68) genomic RNA in wastewater solids was measured longitudinally at two California wastewater treatment plants twice per week for 26 months. EVD68 RNA was undetectable except when concentrations increased between mid-July and mid-December 2022, during which time EVD68 cases were confirmed in the state. ### Competing Interest Statement B. White, D. Duong, and B. Hughes are employees of Verily Life Sciences; other authors declare no competing interests. ### Funding Statement This study was funded by a gift from the Sergey Brin Family Foundation to A. Boehm. ### Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes The details of the IRB/oversight body that provided approval or exemption for the research described are given below: This study was reviewed by the State of California Health and Human Services Agency Committee for the Protection of Human Subjects and determined to be Exempt from oversight. I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable. Yes Wastewater data are publicly available at https://doi.org/10.25740/qt551tn4819.