Bta-miR-484 Targets SFRP1 and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis.

MicroRNAs (miRNAs) are essential regulators of numerous biological processes in animals, including adipogenesis. Despite the abundance of miRNAs associated with adipogenesis, their exact mechanisms of action remain largely unknown. Our study highlights the role of as a major regulator of adipocyte proliferation, apoptosis, and differentiation. Here, we demonstrated that the expression of initially increased during adipogenesis before decreasing. Overexpression of in adipocytes ultimately inhibited cell proliferation and differentiation, reduced the number of EdU fluorescence-stained cells, increased the number of G1 phase cells, reduced the number of G2 and S phase cells, and downregulated the expression of proliferation markers ( and ) and differentiation markers (, , and ). Additionally, overexpression of promoted the expression of apoptosis-related genes (, , and ), and increased the number of apoptotic cells observed via flow cytometry. In contrast, inhibition in adipocytes yielded opposite effects to those observed during overexpression. Moreover, luciferase reporter assays confirmed as a target gene of , and revealed that downregulates mRNA expression. These findings offer compelling evidence that targets , inhibits proliferation and differentiation, and promotes apoptosis. Therefore, these results offer novel insights into the regulation of adipocyte growth and development.