Acclimation Response of Green Microalgae Chlorella Sorokiniana to 2,3′,4,4′,6‐Pentachlorobiphenyl

Abstract The effect of the toxicant 2,3′,4,4′,6‐pentachlorobiphenyl (PCB‐119) on the growth, chlorophyll content, and PSII activity of C. sorokiniana cells was investigated. A strong negative effect of the toxicant was observed at PCB concentration of 0.05 μg mL −1 , when culture growth ceased, chlorophyll strongly bleached, and cell death occurred. The use of original highly sensitive fluorimeter to measure three types of high‐resolution chlorophyll fluorescence kinetics allowed us to detect an initial dramatic decrease in the activity of primary photosynthetic reactions, followed by their almost complete recovery at the end of the incubation period when most cells were dead. The study of the distribution of individual cells in culture in terms of F v / F m parameter, which reflects the quantum yield of PSII photochemistry, revealed the existence of 2–3% of cells retaining high F v / F m (>0.7) in the presence of the toxicant. The treated cultures were able to resume growth after prolonged incubation in fresh medium. The high sensitivity fluorescence methods used made it possible to identify stress‐resistant cells which maintain high photosynthetic activity in the presence of lethal doses of toxic substances; these cells provide recovery of the population after stress.