Staphylococcus aureusColony Polymerase Chain Reaction
CSH Protocols(2023)
摘要
Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.
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关键词
aureus</i>colony polymerase chain reaction,<i>staphylococcus aureus</i>colony
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