Mp01-13 e-cigarette vapor exposure negatively affects spermatogenesis in mice

You have accessJournal of UrologyCME1 Apr 2023MP01-13 E-CIGARETTE VAPOR EXPOSURE NEGATIVELY AFFECTS SPERMATOGENESIS IN MICE Daniel Pelzman, Patrick Walsh, Tatum Tarin, Miguel Brieño-Enriquez, and Kathleen Hwang Daniel PelzmanDaniel Pelzman More articles by this author , Patrick WalshPatrick Walsh More articles by this author , Tatum TarinTatum Tarin More articles by this author , Miguel Brieño-EnriquezMiguel Brieño-Enriquez More articles by this author , and Kathleen HwangKathleen Hwang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003212.13AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Vaping, or e-cigarette usage, has grown drastically in popularity over the past decade, particularly among teenagers and young adults. The impact of vaping on spermatogenesis has not been established. The primary aim of our study was to assess the effects of e-cigarette vapor exposure on semen parameters, patterns of testicular apoptosis, and meiotic function in mice. METHODS: Outbred CD-1 mice were divided into five cohorts: 5-week experimental, 10-week experimental, 5-week control, 10-week control, and wild-type. Experimental groups were subjected to 200 puffs of e-cigarette vapor over 100 minutes 5 times per week for 5 and 10 weeks. Control groups underwent the same protocol but with 100% glycerol as the aerosolized vapor. Wild type mice were not exposed to either protocol. After completing the protocol, the mice were sacrificed and testes/epididymides were harvested. Epididymal sperm concentration was calculated, TUNEL assay was performed on testicular sections, and meiotic spreads were performed on testicular tissue. The expression and localization of MLH1, RPA, MEIOB, SYCP1, and H2AX were assessed using immunohistochemical staining with >50 cells analyzed per mouse. RESULTS: Epididymal sperm concentration was decreased in both control and experimental groups when compared to wild-type (Figure 1). There were higher numbers of apoptotic cells per seminiferous tubule in the control and experimental groups compared to wild-type (Figure 2). Fewer MLH1 foci per cell and increased autosomal H2AX localization were seen in experimental and control groups compared to wild-type, indicating abnormal DNA repair. CONCLUSIONS: Exposure to both aerosolized e-cigarette fluid and 100% glycerol negatively impacted spermatogenesis in mice, possibly via abnormal apoptotic pathways or DNA repair mechanisms. Source of Funding: Funding was provided internally by the UPMC Department of Urology. © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e7 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Daniel Pelzman More articles by this author Patrick Walsh More articles by this author Tatum Tarin More articles by this author Miguel Brieño-Enriquez More articles by this author Kathleen Hwang More articles by this author Expand All Advertisement PDF downloadLoading ...