Mp01-10 a novel and feasible method for quantifying testicular speramatogenesis by using creatine chemical exchange saturation transfer imaging

You have accessJournal of UrologyCME1 Apr 2023MP01-10 A NOVEL AND FEASIBLE METHOD FOR QUANTIFYING TESTICULAR SPERAMATOGENESIS BY USING CREATINE CHEMICAL EXCHANGE SATURATION TRANSFER IMAGING Sohei Kuribayashi, Shinichiro Fukuhara, Go Tsujimura, Takahiro Imanaka, Koichi Okada, Norichika Ueda, Kentaro Takezawa, Shigeyoshi Saito, Yusuke Takahashi, Hidetaka Kioka, and Norio Nonomura Sohei KuribayashiSohei Kuribayashi More articles by this author , Shinichiro FukuharaShinichiro Fukuhara More articles by this author , Go TsujimuraGo Tsujimura More articles by this author , Takahiro ImanakaTakahiro Imanaka More articles by this author , Koichi OkadaKoichi Okada More articles by this author , Norichika UedaNorichika Ueda More articles by this author , Kentaro TakezawaKentaro Takezawa More articles by this author , Shigeyoshi SaitoShigeyoshi Saito More articles by this author , Yusuke TakahashiYusuke Takahashi More articles by this author , Hidetaka KiokaHidetaka Kioka More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003212.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The sperm retrieval rate in non-obstructive azoospermia (NOA) patients is still insufficient. To overcome this problem, an accurate noninvasive method of evaluating testicular spermatogenesis is needed. Chemical exchange saturation transfer (CEST) imaging is a new magnetic resonance imaging (MRI) technique that can image the distribution of trace substances in vivo. We focused on the potential role of creatine (Cr) in testes and hypothesized that Cr-CEST could indicate intratesticular spermatogenesis noninvasively. METHODS: We investigated the Cr-CEST signal in the testes of the testicular ischemia model and the partial testicular irradiation model. In the testicular ischemia model, we clamped the right spermatic cord from 6–42 hours. To evaluate spatial resolution, we performed Cr-CEST for a partial testicular irradiation model in which the lower half of the testes was irradiated with a single 6 Gy dose. RESULTS: In the testicular ischemia models, eight testicular ischemia models were serially imaged at two or three-time points and a total of 26 images of ischemic testis were obtained. The signal intensity of ischemic testis was significantly lower than that of controls (4.0±2.0 vs. 8.9±0.6, p<0.01, Figure 1). Correlation analysis revealed a strong linear correlation between the signal intensity decline and the duration of ischemia (r=0.96, p<0.01, Figure 1). In the partial irradiation model, the signal intensity in the irradiated area was significantly lower than that in the non-irradiated area (5.3± 1.4 vs 7.6±0.3, p<0.05, Figure 1). CONCLUSIONS: Cr-CEST noninvasively evaluate intratesticular spermatogenesis both qualitatively and spatially from this study. Cr-CEST would be used as feasible preoperative imaging for evaluating intratesticular spermatogenesis and a navigation system during microscopic intratesticular sperm extraction. Source of Funding: This research was supported by the Japan Agency for Medical Research and Development (AMED) (grant number: J210705035) and Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (grant number: A21H030670) © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e5 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sohei Kuribayashi More articles by this author Shinichiro Fukuhara More articles by this author Go Tsujimura More articles by this author Takahiro Imanaka More articles by this author Koichi Okada More articles by this author Norichika Ueda More articles by this author Kentaro Takezawa More articles by this author Shigeyoshi Saito More articles by this author Yusuke Takahashi More articles by this author Hidetaka Kioka More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...