Synthesis and anthelmintic activity of novel thiosemicarbazide and 1,2,4-triazole derivatives: In vitro, in vivo, and in silico study

•New thiosemicarbazide and 1,2,4-triazole derivatives, not described in the literature, were synthesized.•The anthelmintic activity was tested in vitro and in vivo on four pathogenic nematode species.•The in vivo studies showed no systemic toxicity of the tested compound (II-1).•The degree of inhibition of selected CYP450 enzymes depending on the concentration of the tested compound II-1 was assessed.•The in silico studies indicated tubulin and the SDH enzyme as potential molecular targets of the tested compound (II-1)