Importancia de los cultivos celulares. Optimización de un ensayo para evaluar el impacto biológico del cannabidiol (CBD)

Primary cell cultures and cell lines are part of the fundamental tools for the evaluation of the efficacy and cytotoxicity of drugs, protein expression, vaccine production, evaluation of the pathogen-host relationship, among other versatile applications. Cellular study models are used to simulate dental, periodontal, and craniofacial diseases. However, the optimization and standardization can result in multiple errors that can lead to inappropriate interpretations of the results obtained in drug evaluation. This article has detailed the basic aspects of cell cultures, reagent quality control measures and good practices, the most frequent mistakes committed when culturing cells and the applicable prevention methods to reduce the risk of error. Likewise, a pilot study of the biological impact of cannabidiol (CBD) in culture with human stem cells (hDPSC) is detailed, highlighting it’s potential therapeutic effect in dentistry.