Recent Progress in Fabrication of Electrospun Nanofiber Membranes for Developing Physiological in vitro Organ/Tissue Models.

Nanofiber membranes (NFMs), which have an extracellular matrix (ECM)-mimicking structure and unique physical properties, have garnered great attention as biomimetic materials for developing physiologically relevant in vitro organ/tissue models. Recent progress in NFM fabrication techniques immensely contributed to the development of NFM-based cell culture platforms for constructing physiological organ/tissue models. However, despite the significance of the NFM fabrication technique, an in-depth discussion of the fabrication technique and its future aspect was insufficient. This review provides an overview of the current state-of-the-art of NFM fabrication techniques from electrospinning techniques to post-processing techniques for the fabrication of various types of NFM-based cell culture platforms. Moreover, the advantages of the NFM-based culture platforms in the construction of organ/tissue models are discussed especially for tissue barrier models, spheroids/organoids, and biomimetic organ/tissue constructs. Finally, the review concludes with perspectives on challenges and future directions for fabrication and utilization of NFMs. This article is protected by copyright. All rights reserved.