Cell-Free Expression Systems: Methods of Production and Use.

Journal of visualized experiments : JoVE(2023)

引用 0|浏览3
暂无评分
摘要
Grasemann, L., Lavickova, B., Elizondo-Cantu, M. C., Maerkl, S. J. OnePot PURE cell-free system. Journal of Visualized Experiments. (172), doi:10.3791/62625 (2021). Smith, P. E. J., Slouka, T., Dabbas, M., Oza, J. P. From cells to cell-free protein synthesis within 24 hours using cell-free autoinduction workflow. Journal of Visualized Experiments. (173), doi:10.3791/62866 (2021). Cooper, R. M., Tonooka, T., Didovyk, A., Hasty, J. Rapid, affordable, and uncomplicated production of bacterial cell-free lysate. Journal of Visualized Experiments. (176), doi:10.3791/62753 (2021). Dopp, J. L., Reuel, N. F. Rapid, enzymatic methods for amplification of minimal, linear templates for protein prototyping using cell-free systems. Journal of Visualized Experiments. (172), doi:10.3791/62728 (2021). McManus, J. B. et al. Rapid characterization of genetic parts with cell-free systems. Journal of Visualized Experiments. (174), doi:10.3791/62816 (2021). Toh, M., Chengan, K., Hanson, T., Freemont, P. S., Moore, S. J. A high-yield streptomyces transcription-translation toolkit for synthetic biology and natural product applications. Journal of Visualized Experiments. (175), doi:10.3791/63012 (2021). Dinglasan, J. L. N., Reeves, D. T., Hettich, R. L., Doktycz, M. J. Liquid chromatography coupled to refractive index or mass spectrometric detection for metabolite profiling in lysate-based cell-free systems. Journal of Visualized Experiments. (175), Drachuk, I., Harbaugh, S., Kelley-Loughnane, N., Chavez, J. L. Preparation of multifunctional silk-based microcapsules loaded with DNA plasmids encoding RNA aptamers and riboswitches. Journal of Visualized Experiments. (176), doi:10.3791/62854 (2021). Jaenes, K. et al. Design to implementation study for development and patient validation of paper-based toehold switch diagnostics. Journal of Visualized Experiments. (184), doi:10.3791/63223 (2022).
更多
查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要