Flow Cytometric Analysis of Regulated Cell Death.

Cell death is a crucial and fundamental process in the biology of all living organisms and plays an essential role in developmental, cellular, and molecular biology. With a deeper understanding of the mechanisms of different types of cell death, quantitative experiments are becoming increasingly necessary to analyze the dynamic changes and coordinate physiological processes. Flow cytometry is the most widely used method for detecting and quantifying cell processes in mammalian cells, providing a comprehensive and high-throughput approach, even at the single-cell level. This chapter provides a brief overview of guidelines for performing flow cytometry in the detection of regulated cell deaths, including apoptosis, autophagy, ferroptosis, pyroptosis, and immunogenic cell death.