Investigating the Role of FoxP3 in Renal Cell Carcinoma Metastasis with BAP1 or SEDT2 Mutation.

Forkhead box protein P3 () primarily functions as the master regulator in regulatory T cells (Tregs) differentiation, but its high level of expression has also been found in tumor cells recently. The aim of our study was to clarify the role of in renal cell carcinoma (RCC) progression and metastasis. We verified the characteristic clinicopathological data from The Cancer Genome Atlas (TCGA) database using bioinformatics tools. Meanwhile, RNA sequencing was performed to determine the biofunction in RCC progression. Our results showed that high expression of was found in - or -mutant patients with RCC, and a higher expression was related to worse prognosis. However, there was no statistically significant relationship between the IHC score and RCC malignant progression owning to the limited number of patients in our tissue microarray. Using in vitro loss-of-function assays, we verified that silencing in 786-O and ACHN cells could inhibit the cell migration/invasion capability, which was consistent with the data from RNA sequencing in 786-O cells and from the TCGA datasets. Using an in vivo nude mice orthotopic kidney cancer model, we found that silencing could inhibit tumor growth. In conclusion, our study demonstrated that or mutation could lead to higher expression of in RCC patients, and could eventually stimulate RCC cells' invasion and metastasis, which might indicate that could function as a potential oncogene in RCC progression.