Cuttlefish Species Authentication: Advancing Label Control through Near-Infrared Spectroscopy as Rapid, Eco-Friendly, and Robust Approach.

Accurate species identification, especially in the fishery sector, is critical for ensuring food safety, consumer protection and to prevent economic losses. In this study, a total of 93 individual frozen-thawed cuttlefish samples from four different species (, , , and ) were collected from two wholesale fish plants in Chioggia, Italy. Species identification was carried out by inspection through morphological features using dichotomic keys and then through near-infrared spectroscopy (NIRS) measurements. The NIRS data were collected using a handled-portable spectrophotometer, and the spectral range scanned was from 900-1680 nm. The collected spectra were processed using principal component analysis for unsupervised analysis and a support vector machine for supervised analysis to evaluate the species identification capability. The results showed that NIRS classification had a high overall accuracy of 93% in identifying the cuttlefish species. This finding highlights the robustness and effectiveness of spectral analysis as a tool for species identification, even in complex spatial contexts. The findings emphasize the potential of NIRS as a valuable tool in the field of fishery product authentication, offering a rapid and eco-friendly approach to species identification in the post-processing stages.