Creation of a device for detecting fluorescence from microfluidic chips

ST PETERSBURG POLYTECHNIC UNIVERSITY JOURNAL-PHYSICS AND MATHEMATICS(2022)

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摘要
In this paper, we consider the creation and testing of a prototype for recording fluorescence from microfluidic chips during the polymerase chain reaction (PCR). The paper presents the characteristics of the main elements used to create the layout of the device for fluorescence detection. The results of experiments in testing the performance of mock-up elements and microfluidic chips are presented. The operability of the assembled layout was demonstrated during the real-time PCR reaction.
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关键词
polymerase chain reaction (PCR), microfluidic chip, DNA, fluorescence, dyes, thermal cycler, optical fiber, amplification
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