Total phenol and flavonoid content, antibacterial and antioxidant activity of extract and fractions of medicinal plants of the Rumex (Polygonaceae) family in the flora of Uzbekistan

In this study, we have determined the antimicrobial, antioxidant activity, the content of phenols and flavonoid in extracts and fractions of plants belonging to the genus Rumex (Polygonaceae), widely distributed in the flora of Uzbekistan. To determine the antimicrobial activity of extracts and fractions, test strains of E. coli, B. subtilis, P. aureginosa, S. aureus, C. albicanc were used. To determine the antioxidant activity, standard solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used. The best results for the total content of phenols and flavonoids in the extracts were as follows: ethanol extract of R. tianschanicus (TPC) 41.23 +/- 4.10 mg GAE/g, (TFC) 58.41 +/- 2.18 mg CE/g. With regard to antimicrobial activity, the following results were obtained. The R. confertus EtOH fraction showed higher antimicrobial properties against E. coli (17 +/- 0.12), B. subtilis (14 +/- 0.17), P. aureginosa (13 +/- 0.10), C. albicans (17 +/- 0.10) compared to other samples. The Rumex pomiricus CHCl3 fraction showed high antimicrobial properties against E. coli (15 +/- 1.21), B. subtilis (13 +/- 1.01), P. aureginosa (20 +/- 0.21), C. albicans (25 +/- 0.13). The results of the minimum inhibitory concentration were as follows: extracts of R. pomiricus MIC (6.25 +/- 0.02 mu g/ml); MIC of R. pomiricus CHC l3 fraction (6.25 +/- 0.107 mu g/ml). In terms of antioxidant activity (DPPH), IC50 values (p<0.01) for ascorbic acid 24.53 mu g/ml, R. confertus EtOH fraction 134.9 mu g/ ml significant changes were noted. R. syriacus leaf extract 70% showed IC50 values (p<0.01) of 48.54 mu g/ml.