High-quality DNA isolation protocol for detection of Khapra beetle (Dermestidae: Trogoderma granarium Everts, 1898) in standard wheat germ trap.

Xiaocheng Zhu,David Gopurenko,Francesca Galea,Ian B Marsh,Sandra McDougall,Agasthya Thotagamuwa

Molecular biology reports（2023）

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摘要

This DNA extraction protocol makes it possible to perform a more rapid identification of the pest following wheat germ sample collection. The protocol has potential to improve international efforts for Khapra beetle surveillance.

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关键词

Quantitative PCR, Grain pest, DNA extraction, Molecular identification, Khapra beetle, Trogoderma granarium

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