iTRAQ based proteomic analysis of rice lines having single or stacked blast resistance genes: Pi54 / Pi54rh during incompatible interaction with Magnaporthe oryzae

Deployment of single or multiple blast resistance ( R ) genes in rice plant is considered to be the most promising approach to enhance resistance against blast disease caused by fungus Magnaporthe oryzae . At the proteome level, relatively little information about R gene mediated defence mechanisms for single and stacking resistance characteristics is available. The overall objective of this study is to look at the proteomics of rice plants that have R genes; Pi54, Pi54rh and stacked Pi54 + Pi54rh in response to rice blast infection. In this study ‘isobaric tag for relative and absolute quantification’ (iTRAQ)-based proteomics analysis was performed in rice plants at 72-h post inoculation with Magnaporthe oryzae and various differentially expressed proteins were identified in these three transgenic lines in comparison to wild type during resistance response to blast pathogen. Through STRING analysis, the observed proteins were further examined to anticipate their linked partners, and it was shown that several defense-related proteins were co-expressed. These proteins can be employed as targets in future rice resistance breeding against Magnaporthe oryzae . The current study is the first to report a proteomics investigation of rice lines that express single blast R gene Pi54 , Pi54rh and stacked ( Pi54 + Pi54rh ) during incompatible interaction with Magnaporthe oryzae . The differentially expressed proteins indicated that secondary metabolites, reactive oxygen species-related proteins, phenylpropanoid, phytohormones and pathogenesis-related proteins have a substantial relationship with the defense response against Magnaporthe oryzae .