Reconfigurable Nanobook Structure Driven by Polymerase-Triggered DNA Strand Displacement.

Due to its high degree of customization, DNA origami provides a versatile platform with which to engineer nanoscale structures and devices. Reconfigurable nanodevices driven by DNA strand displacement accomplish the task of transition between different conformations, endowing DNA origami with application values. Herein, we propose a strategy to regulate the conformation of DNA origami using the polymerase-triggered DNA strand displacement (PTSD) reaction. We design a book-shaped DNA origami structure consisting of four pages connected into a cuboid shape. The PTSD reactions initiated by different primer strands selectively remove the connecting strand, transforming the nano book into a two-page or a four-page conformation. We utilize three primer strands to remove thirty-five connecting strands and construct three conformations of the identical DNA origami, illustrating that the PTSD reaction is an effective tool for the reconfiguration of DNA origami. The statistical results of TEM images prove the effectiveness of the proposed method. Our work on the development of PTSD-driven reconfigurable nanostructure will offer a new way to create intelligent materials for advanced nanotechnology applications.