In vitro modulation of hedgehog signaling in primary hepatocytes

The liver coordinates a variety of pathways and is responsible for lipid, glucose and xenobiotic metabolism. Hedgehog (Hh) signaling is involved in organ homeostasis and regulation of metabolism. When dysregulated, it contributes to various liver diseases such as NAFLD. Hepatocytes, which comprise the parenchyma and therefore the majority of hepatic cells, are a target for therapeutic approaches. As in vitro manipulation of Hh is a part of preclinical trials, our study aims to provide comprehensive data of the effects of assorted modulators on Hh and the interacting Wnt pathway in hepatocytes.