A new HPLC-MS/MS method for urinary creatinine determination: Comparison study with Jaffè’s method

Urinary biomarkers are usually reported as a normalized ratio to creatinuria, a lack of correct creatinine determination lead to wrong final data. This study aimed to evaluate the Jaffè method accuracy, proposing a new HPLC-MS/MS method. The validation parameters were: run time of 4 ​min, LOD of 0.02 ​mg/L, CV% lower than 4%, recovery >96.1%, no matrix effect.