ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Planarian cell dissociation methods using enzymatic approaches are well established and have been widely used in the field. However, their use in transcriptomics and especially single-cell transcriptomics raises concerns as cells are dissociated alive, and this induces cellular stress responses. Here we describe a protocol for planarian cell dissociation using ACME, a dissociation-fixation approach based on acetic acid and methanol. ACME-dissociated cells are fixed, can be cryopreserved, and are amenable to modern methods of single-cell transcriptomics.
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关键词
flow cytometry,cell sorting,cells,dissociation-fixation
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