Non-interference delivery of Ce6 and DOX in NIR light-responsive liposomes for synergetic cervical cancer therapy.

Multi-modal combined treatment of malignant tumors can make up for the shortcomings of single treatment through multi-target and multi-path effects to achieve more ideal tumor treatment effect. However, the mutual interference of different drugs in the delivery process in vivo and the difficulty of effective drug accumulation in tumor cells in the first release are the bottlenecks of combined therapy. To this project, light-responsive liposomes (DOX-Ce6-Lip) loading doxorubicin (DOX) and Chlorin e6 (Ce6) without mutual interference were engineered by thin film hydration method. This kind of nano-drug delivery system increased the drugs concentration accumulated in tumors through the EPR effect, and reduce the toxic and side effects of drugs on other tissues in vivo. In addition, after entering the tumor cells, Ce6 produced a large number of reactive oxygen species (ROS) under 660 nm NIR laser irradiation. These ROS further oxidized the unsaturated fatty acid chain in the liposomes and caused the collapse of the liposomes, thus realizing the stimulus-responsive release of Ce6 and DOX. The concentration of DOX and Ce6 in the tumor rapidly reached the peak and achieved a more effective combination of chemotherapy and photodynamic therapy. Consequently, DOX-Ce6-Lip with 660 nm NIR irradiation achieved an efficient tumor growth inhibition of 71.90 ± 3.14%, indicating the versatile potential of chemotherapy and PDT. .