Evaluating Escherichia coli contamination in bivalve mollusks using the impedance method: a comparison with most probable number analyses and correlation with environmental parameters.

The application of an electrochemical (impedance) tool for monitoring contamination in shellfish was evaluated after 13 months of observation. The primary aim of the present study was to compare the standard most probable number (MPN) and μ-trac 4200 (log imped/100 g) for the assessment of contamination (log MPN/100 g) in non-depurated bivalve mollusks (BM) from five sampling areas of the Veneto-Emilian coast (Italy) (118 samples). The secondary aim was to evaluate the correlation between concentrations in BM and environmental factors on a large data set (690). The methods showed a moderate, positive correlation (0.60 and 0.69 Pearson and Spearman coefficients, respectively; P0.01) in The McNemar test indicated analogous sample classification between methods, and the impedance method overestimated the most contaminated class (P=0.03; >4,600 MPN/100 g). The results highlighted the suitability of the impedance method for a faster evaluation and routine use especially in clams, while in it seemed less effective. Different models built by multivariate permutational variance analysis and multinomial logistic regression selected the suitable environmental features able to predict the load. Overall, salinity and season affected the contamination, whereas locally it was mainly influenced by hydrometry and salinity. The application of the impedance method coupled with environmental data analysis could help purification phase management to adhere to legal limits and could represent an advantage for local control authorities to define actions, considering extreme meteorological events' effects as a proactive reaction to climate change.