Excitation-depended fluorescence emission of boron-doped graphene quantum dot as an optical probe for detection of oxytetracycline in food and information encryption patterns

The boron-doped graphene quantum dot (HSE-GQD-B) was prepared by thermal pyrolysis of the mixture of citric acid, histidine, serine and ethylenediamine and boric acid. The resulting HSE-GQD-B is composed of tiny graphene sheets with an average sheet size of 4.2 ± 0.16 nm and exhibits an excitation-depended fluorescence emission behavior. The HSE-GQD-B produces the strongest blue fluorescence of 450 nm wavelength under the excitation of 365-nm ultraviolet light and the strongest yellow fluorescence of 550-nm wavelength under the excitation of 470-nm visible light. The interaction of HSE-GQD-B with oxytetracycline molecule induces a sensitive blue fluorescence quenching process. Based on this characteristic, a fluorescence method was established for optical detection of oxytetracycline. The analytical method offers a better sensitivity, selectivity, and repeatability compared with previously reported methods. The detection of oxytetracycline attains a wide linear range of 0.02–50 μM and a detection limit of 0.0067 μM. It has been successfully applied to fluorescence detection of oxytetracycline in food samples. In addition, the HSE-GQD-B was also used as a multicolor fluorescence probe for information encryption patterns. Graphical abstract