Chromatin immunoprecipitation and library preparation: a powerful tool to unravel the epigenome.

Chromatin immunoprecipitation (IP) followed by sequencing (ChIP-seq) is a powerful tool used in genome-wide DNA binding sequences for specific proteins, such as transcription factors and histone marks. ChIP-seq helps decipher cell identity by revealing interactions between transcription factors and regulatory DNA elements, as well as the regulation of gene expression during various stages of development, regeneration and disease [1-3]. Chromatin structures are influenced by intra-and extracellular signal transductions that control several processes like gene transcription, DNA replication, histone modifications, DNA repair and other transcription coregulators [2,4,5]. Understanding genome-wide DNA protein interactions and histone modifications is critical for deciphering the chromatin regulatory landscape. ChIP-seq provides a powerful tool to understand and determine the processes regulated by these specific interactions [2,6-8].